Arnt (NM_001037737) Mouse Untagged Clone

CAT#: MC205717

Arnt (untagged) - Mouse aryl hydrocarbon receptor nuclear translocator (Arnt), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arnt
• Synonyms: bHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b; mKIAA4051; W08714
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC012870
  GCGGCGGTAAGGTGCCTAATCTGCGGAGTGGCTCTTCCCTCCCCTCCCCCAGCTCGGTGGCGGCTGCCCC TCCCACCGAGGGTGGCGCAGGGACGGTGCCATCTCGACCATGGCGGCGACTACAGCTAACCCAGAAATGA CATCAGATGTACCATCGCTGGGTCCCACCATTGCTTCTGGAAACCCTGGACCTGGGATTCAAGGTGGAGG AGCTGTTGTACAGAGGGCTATTAAGCGACGGTCAGGGCTGGATTTTGATGATGAAGTAGAAGTGAACACT AAATTTTTGAGATGCGATGATGACCAGATGTGTAATGACAAAGAGCGGTTTGCCAGGTCGGATGATGAGC AGAGCTCTGCGGATAAAGAGAGACTTGCCAGGGAAAATCATAGTGAAATAGAACGGCGGCGACGGAACAA GATGACAGCTTACATCACAGAACTGTCAGACATGGTACCTACATGTAGTGCCCTGGCTCGAAAACCAGAC AAGCTAACCATCTTACGCATGGCCGTTTCTCACATGAAGTCCTTGAGGGGAACTGGCAACACATCTACTG ATGGCTCCTACAAGCCATCTTTCCTCACTGATCAGGAACTGAAACATTTGATCTTGGAGGCAGCAGATGG CTTTCTGTTTATTGTCTCCTGTGAGACTGGACGGGTGGTGTATGTCTCTGACTCAGTGACTCCCGTTTTG AACCAGCCACAGTCTGAATGGTTCGGGAGCACACTGTATGATCAGGTGCACCCAGATGATGTGGATAAAC TTCGAGAGCAGCTCTCTACATCAGAAAATGCCCTAACAGGGCGGGTCCTGGATCTGAAGACTGGAACAGT GAAAAAGGAAGGCCAGCAGTCTTCCATGAGGATGTGCATGGGCTCACGAAGGTCGTTCATCTGCCGCATG AGGTGTGGTACTAGCTCCGTGGACCCTGTTTCCATGAATAGACTGAGCTTTTTGAGGAACAGATGCAGGA ATGGGCTTGGCTCTGTGAAGGAAGGAGAACCTCACTTTGTGGTAGTCCACTGCACAGGCTACATCAAGGC CTGGCCACCAGCAGGTGTCTCCCTCCCAGATGATGACCCAGAGGCTGGCCAGGGGAGCAAATTCTGCCTA GTGGCCATTGGCAGGCTGCAGGTAACTAGTTCTCCCAACTGTACAGACATGAGTAACATTTGTCAGCCAA CAGAGTTCATCTCCCGACACAACATTGAAGGGATATTCACTTTTGTAGACCATCGTTGTGTGGCTACTGT TGGCTACCAGCCACAGGAGCTCTTAGGGAAGAATATTGTAGAATTTTGTCATCCTGAAGACCAACAACTT CTAAGAGACAGCTTTCAGCAGGTGGTGAAATTAAAAGGTCAGGTGCTGTCCGTCATGTTCCGATTCCGAT CTAAGACCCGAGAATGGCTGTGGATGAGAACGAGCTCCTTTACCTTCCAAAACCCTTATTCAGATGAAAT TGAGTATATTATCTGCACCAACACCAATGTGAAGAACTCTAGCCAGGAACCACGGCCTACACTGTCCAAC ACCATCCCAAGGTCACAGCTAGGTCCGACAGCCAATTTATCCCTAGAGATGGGTACAGGGCAGCTGCCAT CCAGGCAGCAGCAGCAGCAGCACACAGAACTGGATATGGTACCAGGAAGAGATGGGCTGGCCAGCTATAA TCATTCCCAGGTTTCTGTCCAGCCTGTGGCAAGTGCAGGATCAGAACACAGCAAGCCCCTTGAGAAGTCA GAAGGTCTCTTTGCACAGGACAGAGATCCAAGGTTTCCAGAAATCTATCCCAGCATCACTGCAGATCAGA GTAAAGGCATCTCCTCCAGCACTGTCCCTGCCACCCAACAGCTGTTCTCCCAGGGCAGCTCATTCCCTCC TAACCCCCGGCCGGCAGAGAATTTCAGGAATAGTGGTCTTACCCCTCCTGTAACCATTGTCCAGCCATCA TCTTCTGCAGGGCAGATACTGGCCCAGATTTCACGTCACTCCAACCCTGCCCAGGGATCAGCGCCGACCT GGACCTCTAGCTCCCGCCCAGGCTTTGCCGCCCAGCAGGTGCCCACCCAGGCTACAGCCAAGACTCGTTC TTCCCAATTTGGTGTGAACAACTTTCAGACTTCTTCCTCCTTCAGTGCTATGTCTCTTCCGGGTGCTCCC ACTGCCTCATCTGGTACTGCTGCCTACCCTGCTCTCCCCAACCGTGGCTCCAACTTTCCTCCTGAGACTG GACAGACCACAGGACAGTTCCAGGCCCGGACAGCAGAGGGCGTGGGTGTCTGGCCACAGTGGCAGGGCCA GCAGCCCCATCATCGGTCTAGTTCCAGTGAGCAGCATGTTCAGCAGACACAAGCACAAGCACCTAGCCAG CCTGAGGTCTTTCAAGAAATGCTGTCCATGCTGGGAGACCAAAGCAACACCTACAACAATGAAGAATTTC CTGATCTAACTATGTTTCCCCCCTTTTCCGAATAGAACTATTGGGGTGAGGATAAGGGTGGGGGGAAATC ACTGTTTGTTTTTAAAAGCAAATCTTTTGTAAACAGAATAAAAGTTCCTCTCCCTTCCCTTCCCTCACCC CTGATATGTACCCTTTCCACCCCTTGACTTGCTGAAGAAACGTTATAGAAGAAATTAAATGAATTTCCCA GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_001037737
• Insert Size: 2376 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012870, AAH12870
• RefSeq Size: 2691 bp
• RefSeq ORF: 2376 bp
• Locus ID: 11863
• UniProt ID: P53762
• Cytogenetics: 3 40.74 cM
• Gene Summary: Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens (By similarity). The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (PubMed:26245371, PubMed:27782878). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28602820).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) is the longer transcript and encodes the longer isoform (a).