Chil1 (NM_007695) Mouse Untagged Clone

CAT#: MC205686

Chi3l1 (untagged) - Mouse chitinase 3-like 1 (Chi3l1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chil1
• Synonyms: AW208766; Brp39; Chi3l1; Gp39
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC005611
  CGGACGCGTGGGCGGACGCGTGGGGCTGGGTACTAGGAGAAGCCATCATGCACACCTCTACTGAAGCCAG GATGGGCATGAGGGCGGCACTGACAGGCTTTGCGGTCCTGATGCTGCTCCAGAGCTGCTCTGCGTACAAG CTGGTCTGCTACTTCACCAGCTGGTCCCAGTACCGGGAAGGCGTTGGAAGCTTCTTACCAGACGCCATCC AACCTTTCCTGTGCACCCACATCATCTACAGCTTTGCCAACATCAGCAGCGACAACATGCTTAGCACATG GGAGTGGAATGACGAGTCGAACTATGACAAGCTGAATAAACTGAAGACCAGAAACACCAACCTGAAGACC CTCCTGTCTGTTGGAGGGTGGAAATTTGGCGAAAAAAGATTTTCCGAGATTGCCTCCAACACTGAGAGAC GCACTGCTTTCGTCCGGTCGGTAGCCCCGTTCCTGCGTTCTTATGGCTTTGATGGGCTGGATCTCGCCTG GCTCTACCCTCGCTTAAGAGACAAGCAGTATTTCTCCACCCTGATCAAGGAACTGAATGCGGAATTCACA AAGGAGGTCCAGCCAGGCAGAGAGAAACTCCTGCTCAGCGCAGCTTTGTCAGCAGGAAAGGTGGCCATTG ACACTGGCTATGACATCGCCCAGATAGCCCAACACCTGGATTTTATCAATCTCATGACCTACGATTTCCA TGGAGTCTGGCGCCAAATCACAGGCCACCACAGCCCCCTCTTCCAAGGCCAGAAGGACACTAGGTTTGAC AGATACAGCAATGTGAACTATGCCGTGCAGTACATGATACGTCTGGGAGCCCAGGCCAGCAAGCTACTGA TGGGCATCCCCACCTTTGGGAAGAGCTTCACTCTGGCATCTTCTGAAAATCAGTTGGGAGCTCCAATCTC AGGGGAAGGATTACCAGGCCGGTTCACCAAGGAGGCAGGGACCCTGGCCTACTACGAGATATGCGACTTC CTCAAAGGAGCTGAAGTACATCGACTCTCCAACGAGAAGGTTCCCTTCGCTACCAAGGGCAACCAGTGGG TGGGGTATGAGGACAAGGAGAGTGTCAAAAACAAGGTTGGGTTCCTGAAGGAGAAGAAGCTGGCAGGAGC CATGGTGTGGGCACTGGATTTGGATGATTTCCAGGGCACCTGTCAGCCGAAGGAATTCTTCCCGCTCACC AACGCCATCAAGGATGCCCTGGCTTAGCTCCCCCTTTCCCATATGGTACCCCCACTCTCTGGCCAGGAGT TTAATCTCTTGCAATGTTAAGTTCCCCAACTGAGCCTCAGTTTCTCCTTCCCTTGGCACCTGTGTAAGGG GCCACAGCAGGCTCAGCTATGGAGAACAGGGAACTAGGGTAGGACGATGGTGGGGTTGTGAGAGTCACAG TGTGAGCAGATACACAACCCTGTTAAGGAATGCAAATTCTCAGACTCTAACCTCCCTTTACCCAGCCTGA CCAAAGGACACCACTTGGATCAAGTAGGCAAATATCTTACAGGATTGAGGGACCATACTAATTATACCCT CTGCAAAGCCCAACTTGAATCCTTCCCTTAGGAACTTAATCGTCCCACTTCCCTTTCCCTAATTCCACAG CTGTTCAATAAAGCGCCAGAACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007695
• Insert Size: 1146 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005611, AAH05611
• RefSeq Size: 1664 bp
• RefSeq ORF: 1146 bp
• Locus ID: 12654
• UniProt ID: Q61362
• Cytogenetics: 1 58.15 cM
• Gene Summary: Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.[UniProtKB/Swiss-Prot Function]