Map2k6 (NM_011943) Mouse Untagged Clone

CAT#: MC205314

Map2k6 (untagged) - Mouse mitogen-activated protein kinase kinase 6 (Map2k6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Map2k6
• Synonyms: MEK6; MKK6; Prkmk6; SAPKK3
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC075652
  TACCATTCTGATGTTGGAGCGGCCGCCTGCCCTGGCCCATCATGTAGCTGCAGCACAGCCTTCCCTAACG TTGCAACTGGGGGAAAAATCACTTTCTAGACTGTTTTGCAAGGTGTGCAATTCCACCTTGACTCCCTGAA AGTCCATCTGCTGCATCGGTCAAGAGAAACTCCACTTGCATGAAGATTGCACGCCTGCAGCTTGCATCTT TGTTGCAAAACTAGCTACAGAAGAGAAGCAAGGCAAAGTCTTTTGTGCTCCCCTCCCCCATCAAAGGAAA GGGGAAAATGTCTCAGTCGAAAGGCAAGAAGCGAAACCCGGGCCTTAAGATTCCAAAAGAAGCGTTTGAA CAGCCTCAGACCAGTTCCACGCCGCCTCGGGATTTAGACTCCAAGGCTTGCATATCTATTGGAAACCAGA ACTTTGAGGTGAAGGCAGATGACCTGGAGCCGATAGTGGAGCTGGGACGAGGTGCGTACGGGGTGGTGGA GAAGATGCGTCACGTGCCCAGCGGGCAGATCATGGCAGTGAAGCGGATACGGGCCACAGTTAATAGCCAG GAACAGAAACGGCTGCTGATGGATTTGGATGTCTCCATGAGGACGGTGGACTGTCCATTCACCGTGACCT TCTACGGTGCACTCTTCCGGGAGGGCGACGTGTGGATCTGCATGGAGCTCATGGATACGTCACTAGATAA ATTCTACAAACAAGTTATTGATAAAGGCCAAACAATTCCAGAGGATATCTTAGGAAAGATAGCAGTTTCT ATTGTAAAAGCGTTAGAACATTTACACAGTAAGCTGTCTGTTATCCATCGAGACGTCAAGCCTTCTAATG TGCTCATTAACACACTGGGCCAGGTGAAGATGTGTGACTTTGGAATCAGTGGCTACCTGGTCGACTCTGT TGCTAAAACGATCGATGCCGGTTGCAAACCATACATGGCTCCTGAACGAATAAATCCAGAGCTCAACCAG AAGGGGTACAGTGTGAAGTCTGACATTTGGAGCCTGGGCATCACCATGATCGAGCTGGCCATCCTTCGGT TTCCTTATGATTCTTGGGGAACGCCCTTCCAGCAGCTAAAGCAGGTGGTCGAAGAGCCCTCTCCTCAGCT CCCAGCAGACAAGTTCTCCGCGGACTTTGTTGACTTTACCTCACAGTGCTTGAAGAAAAATTCCAAAGAA CGGCCCACATATCCAGAGCTTATGCAACATCCATTTTTCACCGTACATGAATCCAAAGCAGCAGACGTGG CATCTTTTGTAAAACTGATACTTGGGGACTAAAAAGCCATGGACTTAACTGGTCGACCCTACTGTGGATT GGTGGGTTTACAGGGTGAAGAAAGTTCACTACAGAGCCAACAGAAAGTCATCTTGAGGTCATTGAACCCT GCCTTTCTGAGGGGTTTCCTCTCCCCGTTTTTCTTTTTCCTCTCCAAAGGGGGCCTTGGAATCTCTAGCG TAGGCTGAACTCTCTAGATGGATGAAATACAACAAAGGCTTAGGACTTGAAATGGTGATTAAATATTTAA TGGCAAGTCATACGGGTGGGTCCTCGAGCTTCTCAGATCTCTCGTGTTCTTTACGAAATGAATGCAATTG GCCCTGGTAACAAGGTGCTACAGTAGTGAAGAGATTGTGAAGTAGATTTGTAGCGTATCCCACTTATTAT TTTAATATTTATGTTTCAGTGCTTGGTTGGAAATATTCCATTTTATGCAAGAAGGGAGATACAGAGACAG GGCTGACTCGGCAGTATTTATAGGGCTTTTATTTTTTTTGAGTTCAATCATGTCTGTGGTCCAGAGGAAG TTATTTAATATGCATTTTTAAGGATATTATAAAAATCTCCAGCAAAGGGGCTCTTCCTGTACAGTGTGGC TTGCAGCTCTCATGGCTGCTGCTCCCGACTGTCAACTCAACCGTGGCTGATCATCGCATCGTTTGAATGA ACTGTCAAAGTTAATGTCCCCCCGCCTCCCTCCCCCAACTTTGGAAACCATGAAAGTCACTTGTATCACG GCTCAAAGAGTAAAAAAATACAATGGTTCTCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
• Restriction Sites: AscI-NotI
• ACCN: NM_011943
• Insert Size: 1005 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC075652, AAH75652
• RefSeq Size: 2101 bp
• RefSeq ORF: 1005 bp
• Locus ID: 26399
• UniProt ID: P70236
• Cytogenetics: 11 E1- E2
• Gene Summary: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).