Pigf (NM_008838) Mouse Untagged Clone

CAT#: MC204558

Pigf (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class F (Pigf), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pigf
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028862
  GTCTGCGCCTGCGCCAGGGGCCATGCCAGCCTGTAGCACCTGAGGGTGGGAGGCGGTGGTGTCCCCCTTC CCTTCGGCGGGCGGGAGAGTTAGATAGCTACCCAAGAAAACATCATGAAAGATACTGACATCAAGAGACT TCTGTATACCAATCTTTTGTGCGTATTTTCAATTTTCCTAAGCATTTTCATTCCGTCCTTCTTCGTGGAC AACTTCTCAGTATTGGAAGCTCATTTGACCTGGCTGTGTATCTGCTCTGCTTCTGTGACCACTGTCAATC TCTTGTCGTACTTAGTAGTGAAACCGAATGTGTCCTCTAAAAGAAGTTCATTGTCACATAAGGTAACGCG AGCTTTGAAATGCTGTGTCTTCTTCCTTATGTCTTGTTTTCTCCTTCACATCATTTTTGTTCTATATGGA GCACCACTAATCGAGTTGGTGCTGGAAACGTTTTTATTTGCAGTTGTTTTGTCTACTTTTACTACAGTAC CTTGTCTCTGTTTGTTAGGACCAAACCTCAAAGCATGGCTGAGAGTTTTCAGTAGAAATGGAGTTACATC TATTTGGGAGAATAGTCTTCAGATCACTACAATTTCTAGTTTCACAGGAGCGTGGCTTGGAGCATTTCCT ATTCCACTGGATTGGGAAAGACCGTGGCAGGTGTGGCCCATCTCCTGTACACTCGGAGCGACCTTTGGCT ACGTGGCTGGCCTGGTTATCTCGCCACTCTGGATCTACTGGAATAGAAAGCAGCTTACATACAAGAACAA TTAAGAGGAGAAAGGAGAGAAGTTATTTCTCTGTGCAGATTCCATAAGGGCTGTGCAGAAGTTACATGGT CCAAGCCGGGCAGTCCATTTACAGCACTGTTGTTTTCTAAATGTCATTTCAACATGATGTGATTGTAGCT TTTTAACTATGAAACCCCTGAGAGATTGTACCTTCTAGTTGAAATAAAGTATTTATAGTAGAAAAAAAAA AAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008838
• Insert Size: 660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028862, AAH28862
• RefSeq Size: 986 bp
• RefSeq ORF: 660 bp
• Locus ID: 18701
• UniProt ID: O09101
• Cytogenetics: 17 56.9 cM
• Gene Summary: Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.[UniProtKB/Swiss-Prot Function]