Fbxo9 (NM_023605) Mouse Untagged Clone

CAT#: MC203866

Fbxo9 (untagged) - Mouse f-box protein 9 (Fbxo9), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fbxo9
• Synonyms: 9030401P18Rik; AA986398
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC020074
  GCAGAGCAGGCAGCTCCGCCGCTGCGTCCAGACGTCTCCTGCTGGGCCGACGCTCAGCTCTTCGCTCGGT GCTCGGCCTCCGCTGGCGACCGGCGACCCCTCCGCAGCTCGAGTCCCCGGCCGCCGCAGCGCGCCTGCCT CCGGCCCCCTGTCCCGCGAAAATGGCTGCCGTACGCCGGGCCCGCAGTTATTGCCGCTGCCTGGTGCGCT TCTCCGACCGAGAACTCTGCTAAGCCGCGCTGCAGCAGACGGGCAGGAGTAGACACCGGGCAGCCGCCAC CCCTCCGGGAGCCGGGCGCGGGAAGCCCCTCGACTGGGCCGGCCGCCGGCCAGCATGGCAGAAGCGGAGG AAGATTGTCATTCTGATGCTGACAGAGTAGGCGATGAAGGAAATGAGAGTCCGGCTGAGAGAGACCTGCA GGCGCAGCTCCAGATGTTCAGAGCTCAGTGGATGTTTGAACTGACCCCAGGTGTAGGCTCCAGTCATGGA GAAACTCGGCCTTGCAGAGCAGGAAGAAGCTCTATGCTGAAAGCAGCTGCAGACACCAAAGGACGACAGG AACTGGCAAAGGAAGAAAAGGCTCGAGAACTCTTCCTGCAGGCAGTGGAGGAAGAACAAAATGGAGCTCT CTATGAAGCCATCAAGTTCTACCGTAGGGCGATGCAGCTGGTGCCAGACATTGAGTTCAAGATCACTTAC ACCCGGTCTCCAGACGGCGACGGCGTTGGGAGCGGCTACATCGAAGAGAACGAGGACGCCAGCAAGATGG CCGATCTCCTGTCGTACTTCCAGCAGCAGCTCACGTTGCAGGAGTCTGTGCTCAAACTCTGTCAGCCTGA GCTTGAGACCAGTCAGACTCACATATCAGTCCTGCCTATGGAGGTGCTGATGTACATCTTCCGATGGGTG GTATCAAGTGACTTGGACCTCAGATCGTTAGAGCAGTTGTCACTGGTGTGCAGAGGATTCTATATCTGTG CCAGAGACCCTGAAATCTGGCGTCTGGCTTGCTTGAAAGTGTGGGGCAGAAGCTGCATGAAGCTCGTTCC ATACGCGTCCTGGAGAGAGATGTTTCTAGAACGGCCCCGAGTTCGGTTTGATGGAGTGTACATCAGTAAA ACCACGTATATTCGCCAGGGAGAGCAGTCACTTGATGGTTTCTACAGGGCGTGGCACCAAGTGGAATATT ACAGATACATGAGATTCTTTCCTGATGGCCATGTGATGATGTTAACCACCCCCGAGGAGCCTCCGTCCAT CGTTCCCCGGTTAAGAACCAGGAACACCAGAACGGATGCAATTCTGCTGGGTCATTACCGCTTGTCACAA GATGCAGACAATCAGACCAAAGTCTTTGCTGTAATAACTAAGAAAAAAGAAGAAAAGCCACTTGACCATA AATACAGGTATTTTCGTCGTGTTCCTGTCCAAGAGGCAGATCACAGCTTCCATGTGGGACTGCAGCTGTG CTCCAGTGGCCACCAGAGGTTCAACAAACTCATCTGGATCCACCACTCTTGTCACATCACTTACAAAGCA ACTGGTGAGACTGCAGTGAGTGCTTTTGAGATTGACAAGATGTACACGCCCCTGTTGTTCGCCAGAGTGA GGAGCTACACTGCCTTCTCGGAAAGGCCTCTGTAGAGCCACACCAGCATGTCACTGCTGCATGAGCAGAA GCATAGAGCGCTGGGACACCTAAGTTATATGTACATAGACACACGCATGTAGATAGACATGCATATATAC ATACATGGAGTTGGAGCTTACTTTAAGTTGTTGGAATTCCTTTAAGAAGAGTATAAATTATTTTTTTTAT TTAAAAGGATAGTTGATTCCTGGGGTACTTTGTTTTGAAATTAGAAGTTGTTAAGGACACTTTTGTTGTA ATAAATGTTGATGTAGAGTTGAAATCATGTTTTCAAGCAGGTTTCTGAGCAGATCTGTCACATCCACTGC CTTGTGCCTGAGCATCCTCAACACACATAGGTCAGGGAGCACACTGGAGAGTTCTTATTCCCGCATGACT TAGTGGCTTTGCATCATACTCAAATGTGAAACCTGTTCTGGATTTGTTTGAAACTGTTCAATAAAGATCA TAAATAAATGTTTCTTTCAAGGAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_023605
• Insert Size: 1311 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020074, AAH20074
• RefSeq Size: 2138 bp
• RefSeq ORF: 1311 bp
• Locus ID: 71538
• UniProt ID: Q8BK06
• Cytogenetics: 9 E1
• Gene Summary: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes isoform 1.