Smpd1 (NM_011421) Mouse Untagged Clone

CAT#: MC203729

Smpd1 (untagged) - Mouse sphingomyelin phosphodiesterase 1, acid lysosomal (Smpd1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smpd1
• Synonyms: A-SMase; ASM; aSMase; Zn-SMase
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC011304
  CGAAGGGTAATCAGGTGTCTCCAGCGTTACCCGGGCCCCGAGGGCCAGCCAGGGCTTCGGCCTGTGGGGC CTGGAGGGGCAAGCCAGTCCTGTCTACGAGCCTGGCAATGCCCCACCACAGAGCATCATCCGGCCAGGAC CACCTCAGAGCCGGCTGGGAGCAGAGACTGGAGAGGTCCTTACCGGCACCCAGAGTGGGACTCCTTTGGA TGGGGCTGGGCTTGGCGCTGGTTCTGGCTCTGTTTGACACCACGGTTCTTTGGGTTCCTGCCAGAGCTTA TCCTCTTCCTTCTGAAGGCCATTCTGTCAAATTCAGTGCCATAGCGCCGCCGCTCCAGAGTGCCTTTGGG TGGCAGAACCTCACTTGCCCCGCCTGCAAAGTCTTATTCACTGCTCTCAACCATGGGCTGAAGAAGGAGC CCAATGTGGCACGGGTAGGCTCTGTGGCCATCAAGATATGCAAGATGCTGAACATAGCACCACTAGATGT GTGCCAGTCAGCCGTCCATCTCTTTGAGGACGATGTGGTGGAGGTGTGGACACGTTCAGTTCTGAGCCCA TCAGAGGCTTGTGGCTTGCTTTTGGGCTCCTCTTGTGGACACTGGGACATCTTTTCGACTTGGAACATCT CTTTGCCATCAGTGCCGAAGCCACCCCCAAAGCCACCGAGCCCACCAGCCCCCGGTGCCCCTGTCAGCCG TGTCCTCTTCCTTACTGATCTACACTGGGACCATGAGTACCTGGAGGGCACAGACCCTTACTGTGCAGAT CCACTTTGCTGCCGCCGGGGGTCCGGATGGCCACCCAACTCCCAGAAGGGGGCTGGGTTCTGGGGCGAGT ACAGCAAGTGTGACCTGCCCTTGCGAACGCTAGAAAGCCTGTTGAAAGGACTGGGCCCTGCCGGCCCTTT TGAAATGGTGTACTGGACGGGAGATATCCCTGCCCATGATGTCTGGCAACAGTCTCGCCAAGATCAGCTG AGGGCCCTGACCACCATCACAGACCTCGTGAGGAAGTTCTTGGGCCCAGTGCCAGTGTACCCTGCTGTGG GCAACCATGAGAGCACTCCTGTCAATGGCTTCCCTCCTCCCTTCATAAAGGGAAACCAATCTTCACAATG GCTCTATGAAGCCATGGCCAAGGCATGGGAACCCTGGTTACCAGCTGATGCCCTTCACACCCTAAGAATT GGGGGCTTCTATGCCCTCACCCCACGCCCTGGCCTCCGCCTCATCTCTCTCAATATGAATTTTTGTTCCC GTGAGAACTTTTGGCTCTTGATCAACTCCACAGATCCTGCTGGACAACTCCAGTGGCTGGTGGAGGAGCT TCAGGCTGCTGAGAATCGAGGAGACAAAGTGCATATAATTGGCCACATCCCTCCAGGACATTGTCTTAAG AGCTGGAGCTGGAATTATTACAAAATCATAGCCAGGTATGAAAACACTCTGGCCAGTCAGTTCTTTGGCC ACACTCACGTGGATGAGTTTGAGATCTTCTATGATGAGGAAACTCTGAGCCGACCACTAGCTGTAGCCTT CCTGGCGCCCAGTGCTACAACCTTTATCAACCTTAACCCTGGCTACCGAGTTTACCAAATAGATGGAAAC TACCCCGGAAGCTCTCATGTGGTCCTGGACCACGAGACCTACATCTTGAATCTGACCCAGGCCAACGCAG CAGGAGGCACACCAAGCTGGAAGCGCCTCTACAGGGCTCGAGAAACCTATGGACTTCCAGATGCGATGCC TGCCTCCTGGCACAACCTGGTCTACCGCATGAGGGATGATGAGCAACTCTTCCAGACCTTCTGGTTTCTC TACCATAAGGGCCACCCACCTTCAGAGCCCTGCGGCACACCCTGCCGCCTGGCCACTCTGTGTGCCCAGC TCTCAGCCCGAGCAGACAGCCCTGCTTTGTGTCGCCACTTGATGCCCAATGGGAGCCTCCCAGATGCCAA TAGGTTGTGGTCACGCCCCCTGTTGTGCTAGTATTCCATGTTCCAGACTGGGGAAAGTTCATGTATCAGA AAAGGGATACATTCCCGAGTGCTGCTTATCTACCTGAGGCAAAAGCTTCGGGGGAAGGAGCCAGCCAGGC CAGGGAAACAAGCCGCCTCATGAGAGCTTGTTTGGTTACACTGGAGGAGGTTTTGGCTGTCCTACTTATG CCCAGGATCTGGATTACCCCTGGGGCTGATGTCTGACCTGGGCTTTGTCGAGGGCTCAGTGCTCTGGATA GTCAGACAGGAGCTATTGCCCGAGCCTGGGCTAATGGACCTCTGAACCCTGTACTGTTACCTGATCCTGC AAGGCTGTTAAAATAAAGAGACACACTTCAGACTCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011421
• Insert Size: 1884 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC011304, AAH11304
• RefSeq Size: 2360 bp
• RefSeq ORF: 1884 bp
• Locus ID: 20597
• UniProt ID: Q04519
• Cytogenetics: 7 55.9 cM
• Gene Summary: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol.[UniProtKB/Swiss-Prot Function]