Lcat (NM_008490) Mouse Untagged Clone

CAT#: MC201471

Lcat (untagged) - Mouse lecithin cholesterol acyltransferase (Lcat), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lcat
• Synonyms: AI046659; D8Wsu61e
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC028861 sequence for NM_008490
  GGCTGTGATGGGGCTGCCTGGCTCCCCATGGCAGCGGGTGCTGTTGCTGTTGGGGCTACTGCTCCCTCCT GCCACCCCCTTCTGGCTCCTCAATGTGCTCTTCCCCCCGCACACCACGCCCAAGGCTGAACTCAGTAACC ACACACGGCCTGTCATCCTCGTGCCTGGCTGCTTGGGGAATCGGCTAGAAGCCAAGCTGGATAAACCAGA TGTGGTGAACTGGATGTGCTACCGTAAGACAGAGGACTTCTTCACCATCTGGCTGGATTTCAACTTGTTT CTCCCCCTCGGGGTGGACTGCTGGATTGATAATACCAGGATTGTCTACAACCACAGCTCTGGGCGCGTGT CCAATGCCCCTGGTGTCCAGATCCGTGTCCCTGGCTTTGGCAAGACCGAATCTGTTGAGTACGTGGATGA CAACAAGCTAGCAGGCTACCTGCACACACTGGTGCAGAATCTGGTTAACAACGGATATGTGCGGGATGAG ACAGTGCGGGCCGCACCCTATGACTGGCGTCTGGCACCCCACCAGCAGGATGAATACTACAAGAAGCTGG CTGGCCTGGTAGAGGAGATGTATGCCGCTTATGGGAAGCCTGTCTTCCTCATTGGGCATAGCCTTGGCTG TCTGCATGTGCTCCACTTCTTACTGCGGCAGCCTCAGTCCTGGAAGGACCACTTCATTGATGGTTTTATC TCTCTCGGGGCTCCGTGGGGTGGTTCCATCAAGGCCATGCGGATCCTGGCCTCAGGTGACAACCAGGGCA TCCCCATCCTGTCCAACATAAAGCTGAAAGAGGAGCAGCGCATAACCACGACTTCCCCCTGGATGCTCCC AGCCCCTCACGTGTGGCCTGAAGACCATGTGTTCATTTCCACACCAAACTTCAACTACACCGTCCAAGAC TTTGAGCGTTTTTTCACAGATCTGCATTTTGAAGAAGGCTGGCACATGTTTCTTCAGTCTCGTGACCTAC TGGAGCGCCTCCCCGCACCTGGTGTAGAAGTATATTGTCTCTACGGTGTGGGCAGACCCACACCCCACAC CTACATCTATGACCACAACTTCCCCTACAAAGACCCCGTGGCTGCACTCTATGAAGATGGGGACGACACC GTAGCCACCCGCAGCACTGAGCTCTGTGGCCAGTGGCAGGGCCGCCAGTCGCAGCCCGTACATTTGCTGC CCATGAACGAGACAGATCACCTCAACATGGTCTTCAGCAATAAGACACTGGAGCATATCAATGCCATCCT ACTGGGTGCCTACCGCACTCCTAAGTCGCCAGCTGCCAGCCCAAGTCCCCCACCCCCTGAATAAAGACCT AGCTGTTATAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008490
• Insert Size: 1317 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC028861, AAH28861
• RefSeq Size: 1354 bp
• RefSeq ORF: 1317 bp
• Locus ID: 16816
• UniProt ID: P16301
• Cytogenetics: 8 53.06 cM
• Gene Summary: Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed:19065001). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels (PubMed:19065001). Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins (PubMed:19065001). Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed:19065001). Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines (PubMed:8820107).[UniProtKB/Swiss-Prot Function]