Slc22a1 (NM_009202) Mouse Untagged Clone

CAT#: MC200831

Slc22a1 (untagged) - Mouse solute carrier family 22 (organic cation transporter), member 1 (Slc22a1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc22a1
• Synonyms: Lx1; Oct1; Orct; Orct1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC021651 sequence for NM_009202
  CCACGCGTCCGCGGGGTAAAAGGTTGTTTCTGGTTGGTTGCCTTCCAGATGTATCAGGCTTGAACAGCAA CTTGATTTCAAGCCACCGCAGTTCCAAGAACTTTAAATGAGACGCTGCACGAACCCCCAGTGGCAGTCCA GAGCAGGCCTGGCTGAGTTGGTGAGGGCCCTACCCAGCCATGCCCACCGTGGACGATGTCCTGGAGCACG TTGGAGAGTTTGGCTGGTTCCAGAAGCAAGCCTTCCTGTTGCTATGCCTGATCTCAGCTTCTTTAGCTCC CATCTACGTGGGCATCGTTTTCCTGGGCTTCACCCCCGACCACCACTGCCGGAGTCCTGGAGTGGCCGAG CTGAGCCAGCGGTGTGGCTGGAGCCCGGCAGAGGAGCTGAACTACACCGTGCCGGGCCTGGGGTCTGCGG GTGAGGCCTCCTTCCTCAGCCAATGCATGAAGTATGAGGTGGACTGGAACCAGAGCACCCTTGACTGTGT GGACCCACTGTCCAGCCTGGCTGCCAACAGGAGCCACTTGCCACTGAGCCCCTGCGAGCATGGCTGGGTG TACGACACTCCCGGCTCCTCCATCGTCACTGAGTTTAACCTGGTGTGTGGAGACGCCTGGAAAGTGGACC TTTTTCAGTCCTGTGTGAACTTGGGCTTCTTCCTGGGCTCCCTGGTTGTGGGTTACATTGCAGACAGGTT TGGCCGTAAGCTCTGCCTCCTGGTGACCACTCTGGTCACCTCCCTGTCTGGCGTGTTAACAGCGGTGGCC CCAGACTATACATCCATGTTGCTCTTTCGCCTTCTGCAAGGCATGGTCAGCAAAGGCAGCTGGGTGTCTG GCTACACCTTGATCACAGAGTTTGTCGGCTCTGGCTACAGGAGAACGACAGCCATCTTGTACCAGGTGGC CTTCACAGTGGGGCTAGTGGGGCTTGCTGGGGTGGCCTATGCCATTCCAGACTGGCGCTGGCTCCAGCTG GCGGTGTCCCTGCCCACCTTCCTCTTCCTGCTGTATTACTGGTTTGTCCCAGAATCCCCCCGGTGGCTGT TGTCTCAGAAGAGAACCACTCAAGCGGTAAGGATAATGGAGCAAATTGCACAGAAGAACAGGAAGGTGCC CCCTGCTGACCTGAAGATGATGTGCCTTGAGGAAGATGCCTCAGAGAGGCGGAGTCCTTCGTTTGCAGAC CTGTTCCGCACCCCCAGCCTGAGGAAGCACACCCTCATCCTGATGTATCTATGGTTCTCTTGTGCTGTGC TGTACCAGGGCCTCATCATGCATGTGGGAGCCACAGGGGCCAACCTCTACCTGGACTTCTTTTATTCTTC TCTAGTGGAATTCCCTGCGGCCTTTATCATCCTTGTCACCATTGACCGCATTGGCCGCATCTACCCAATA GCGGCATCAAATCTGGTGGCGGGGGCAGCCTGCCTCCTCATGATCTTTATCCCACATGAGCTGCATTGGT TGAATGTGACCCTCGCTTGTCTTGGCCGTATGGGAGCCACCATTGTGTTGCAGATGGTCTGCCTAGTGAA CGCTGAGCTGTACCCTACATTCATCAGGAATCTCGGGATGATGGTGTGCTCTGCCCTGTGTGACCTAGGT GGGATCTTCACCCCCTTCATGGTGTTCAGGCTGATGGAAGTTTGGCAAGCCCTGCCCCTCATTTTGTTTG GGGTTTTGGGCCTGAGTGCTGGGGCTGTGACCCTTCTACTTCCGGAGACCAAGGGCGTGGCTCTGCCTGA GACTATTGAAGAAGCGGAGAACCTGGGAAGGAGGAAATCAAAGGCCAAAGAAAACACGATTTACCTTCAG GTCCAAACAGGCAAATCCCCACATACCTGACAGGGATGCCGTGTCAGGAGCTGAGTGGAGAGAGGAAGGA GGACTGGCCACTTGGAAGATTCCTAGAAGCCTTTGCCTTCCCAGACACTTCTATATATTCACCAGGTTCC AAATGAATTCCCAACCTTAAAGACTTTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
• Restriction Sites: RsrII-NotI
• ACCN: NM_009202
• Insert Size: 1671 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021651, AAH21651
• RefSeq Size: 2031 bp
• RefSeq ORF: 1671 bp
• Locus ID: 20517
• UniProt ID: O08966
• Cytogenetics: 17 8.63 cM
• Gene Summary: Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)-N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.[UniProtKB/Swiss-Prot Function]