Human SIX3 (NM_005413) AAV Particle

CAT#: RC216286A1V

AAV ORF Particles, serotype AAV-2, SIX3 (Myc-DDK-tagged)-Human SIX homeobox 3 (SIX3), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: SIX3
• Synonyms: HPE2
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216286 representing NM_005413
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTATTCCGCTCCCCCCTAGACCTCTATTCCTCCCACTTCTTGTTGCCAAACTTCGCCGATTCTCACC
  ACCGCTCCATACTTCTGGCGAGTAGCGGCGGCGGGAACGGTGCGGGAGGCGGCGGCGGCGCGGGAGGCGG
  CAGCGGCGGCGGGAACGGTGCGGGAGGCGGCGGTGCTGGCGGAGCAGGCGGCGGCGGCGGCGGCGGCTCC
  AGGGCCCCCCCGGAAGAGTTGTCCATGTTCCAGCTGCCCACCCTCAACTTCTCGCCGGAGCAGGTGGCCA
  GCGTCTGTGAGACGCTGGAGGAGACGGGCGACATCGAGCGGCTGGGCCGCTTCCTCTGGTCGCTGCCCGT
  GGCCCCCGGGGCGTGCGAGGCCATCAACAAACACGAGTCGATCCTGCGCGCGCGCGCCGTGGTCGCCTTC
  CACACGGGCAACTTCCGCGACCTCTACCACATCCTTGAGAACCACAAGTTCACCAAGGAGTCTCACGGCA
  AGCTGCAGGCCATGTGGCTCGAGGCGCACTACCAGGAGGCCGAGAAGCTGCGCGGCCGCCCACTCGGCCC
  GGTGGACAAGTACCGCGTGCGCAAGAAGTTCCCGCTGCCACGCACCATCTGGGACGGCGAGCAGAAGACG
  CATTGCTTCAAGGAGCGGACTCGGAGCCTGTTGCGGGAGTGGTACCTACAGGACCCCTACCCCAACCCCA
  GCAAGAAACGCGAACTGGCGCAGGCCACCGGCCTCACTCCCACACAAGTAGGCAACTGGTTTAAGAACCG
  GCGGCAGCGCGACCGCGCCGCGGCGGCCAAGAACAGGCTCCAGCACCAGGCCATTGGACCGAGCGGCATG
  CGCTCGCTGGCCGAGCCCGGCTGCCCCACGCACGGCTCGGCAGAGTCGCCGTCCACGGCGGCCAGCCCGA
  CCACCAGCGTGTCCAGCCTGACGGAGCGCGCGGACACCGGCACCTCCATCCTCTCGGTAACCTCCAGCGA
  CTCGGAATGTGATGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216286 representing NM_005413
  Red=Cloning site Green=Tags(s)
  
  MVFRSPLDLYSSHFLLPNFADSHHRSILLASSGGGNGAGGGGGAGGGSGGGNGAGGGGAGGAGGGGGGGS
  RAPPEELSMFQLPTLNFSPEQVASVCETLEETGDIERLGRFLWSLPVAPGACEAINKHESILRARAVVAF
  HTGNFRDLYHILENHKFTKESHGKLQAMWLEAHYQEAEKLRGRPLGPVDKYRVRKKFPLPRTIWDGEQKT
  HCFKERTRSLLREWYLQDPYPNPSKKRELAQATGLTPTQVGNWFKNRRQRDRAAAAKNRLQHQAIGPSGM
  RSLAEPGCPTHGSAESPSTAASPTTSVSSLTERADTGTSILSVTSSDSECDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_005413
• ORF Size: 996 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_005413.1, NP_005404.1
• RefSeq Size: 1926 bp
• RefSeq ORF: 999 bp
• Locus ID: 6496
• UniProt ID: O95343
• Cytogenetics: 2p21
• MW: 35.3 kDa