MCAF2 (ATF7IP2) (NM_024997) Human 3' UTR Clone

CAT#: SC214398

3' UTR clone of activating transcription factor 7 interacting protein 2 (ATF7IP2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ATF7IP2
• Synonyms: MCAF2
• ACCN: NM_024997
• Insert Size: 1419 bp
• Sequence Data:
  Insert Sequence

  >SC214398 3’UTR clone of NM_024997
  The sequence shown below is from the reference sequence of NM_024997. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ATCCCTGGGTTTTCTGAAAATCTTACGTAAAAGGTGTTTAATAATGATATACTACTTTTTTTTTCATAT
  TTGTTTGTTTGCAATGTTACTGTAATACTATTTGCCATTGTAAAAGGCCAGCTCAGATTGTGAGCCCTT
  TCTATTGGGACAGTCCTCTTCTATATGTTTTAAGTGGCCAGTAATTTAATGAATTTCTGGTTTGTATTA
  AATATGTCCTTCCAATGGATAAGTTCTAAAACATACGCTATCATTGGCCCATGTTGCTGAGGTGCATTG
  TGAACAATACCTTTAGTGACTGTGGAACTGCTGCTTCTATCAGAAGACCTAGGATACAAGCAGCCACTG
  TTTCCTCCGTTAACAATCAGCTTTTAGTAACCTGCTGTGGTCAGCATAGCTACAGGAAAAGTCAGCCCT
  TGACTGAGGGCCAGATCATCCCTGGAGTGCACCTCTACTAAGATTTTATGAAAAGATGACATGTTGGGC
  TGCATGATAAAAGTTAATTATAAAAATTAAAAGATTTTTTTTTTTGAGATGGAATATTGCTCTGTTGCC
  CAGGCTGGAGTACAGTGGTCACTGCAACCTCCATCTCCCTGGTTCAAGCAATTCTCCTATGTCAGCCTC
  CCAAGTAGCTGGGACTATAGTCACTTGCCACCATGCCCGGCTAATTTATTTTTAGTAGAGATGAGGTTT
  CACCTTGTTGGTCAGGCTGGTCTCAAACTCCTGACCTCAGGGGATCCACCCGCCTCGGCCTCCCGAAGT
  GCTGGGATTATAGGCGTAAGCCACCATGCCTGGCCAAAAATTAAAAGATTTTATGAAACGAAATGGCTT
  CCCATTCTTTCTATTCAGCTTCTAAGTGGGCTATTCATTTGTGCTCTCCTCTTTTTTGTTTTGTTTTGC
  ACTTTGTCAATCATTTGGGGAAATAGCCTGGAGGTCTTTCCTGAATCTGTTTGTAGACATAATAAAATT
  TGTGTGCTGTGTACACAAAAACATAATGTATCTTATGAAAGCATTACCCAACCTGTTTGAGTTGAGAAT
  GCATTTGTCCACCTTTGATTTGAGGCTTTAGTTTGCTGATTTCCTAAATGCTACCCTTTGATCATTTCC
  TGGCCACCATCACAATACTAAGGGGCTCAGATGTGTCTTGTGCCCACCTCTTCCTGAGAAGAGATGGAA
  GTGGAGCTGTGACTAGTACAAGCAGCCCAAGAAACTCTGAACGGGGCCCAATGGAGGCAAACTTGAGCA
  AATAATTGGGATGATAAGAAACAAAAATAATCCCAACTGTTTGAAAGTTCGAAAGAGGGGCATTCTTTT
  CTGGTACATGGTGAGAGTTCAGGCTCCAGAGACTCAAAACGGAATGGTTTTCCTTGGCATTTTGTAAAT
  GCTCTCACATCTGTAGCAATAAAGCTGTTATTTTCTGCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_024997.5
• Summary: Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. The complex formed with MBD1 and SETDB1 represses transcription and probably couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3) activity (Probable).[UniProtKB/Swiss-Prot Function]
• Locus ID: 80063
• MW: 53.2