4 hours incubations; 1 hour washing and analyzing samples
Signal
Colorimetric
Curve Range
62.5pg/ml-5000pg/ml
Sample Type
Human serum, plasma and other biological fluids.
Sample Volume
100µl
Specificity
This kit is used for quantitative detection of Human MAGEA3
Sensitivity
12.9pg/ml
Reactivity
Human
Cross Reactivity
Recombinant human MAGEA6 cross-reacts approximately 10.8 % in this assay. There is no significant cross-reactivity with other relevant proteins.
Interference
No significant interference observed with available related molecules.
Components
MAGEA3 Antibody Coated 96-well Plate in foil pouch with desiccant | 1 plate
Human Native MAGEA3 Standard (250ng/ml) | 0.1 mL
100x Biotinylated Human MAGEA3 Detection Antibody | 0.12 mL
100x Peroxidase Conjugate | 0.12 mL
Assay Buffer | 30 mL
Standard Diluent | 10 mL
Sample Diluent | 30 mL
Wash Buffer Concentrate 20X | 60 mL
TMB Substrate | 12 mL
Stop Solution | 12 mL
Plate Sealer | 3 pieces
Background
Melanoma-associated antigen 3 (MAGE-A3) is broadly expressed in a variety of malignancies such as breast cancer, melanoma, head and neck cancer, lung cancer, gastric cancer, colorectal cancer, and prostate cancer. It has been shown that MAGE-A3 expression is associated with cancer progression and metastasis. In addition, increased expression of MAGE-A3 in cancer cells is associated with poor prognosis of cancer patients.
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in shipping.