Human HSD17B13 ELISA KIT (1 x 96 wells)

SKU EA200008

Human HSD17B13 ELISA KIT (1 x 96 wells)

Product Data

• Species: Human
• Format: 8x12 divisible strips
• Assay Type: Sandwich
• Assay Length: 3.5 hours incubations; 1 hour washing and analyzing samples
• Signal: Colorimetric
• Curve Range: 0.31ng/ml-20ng/ml
• Sample Type: Human cell lysates, serumm and plasma
• Sample Volume: 100µl
• Specificity: This kit is used for quantitative detection of Human HSD17B13
• Sensitivity: 0.1ng/ml
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • HSD17B13 Antibody Coated 96-well Plate in foil pouch with desiccant | 1 plate
  • Recombinant Human HSD17B13 Standard (1000ng/ml) | 0.1 mL
  • 100x Biotinylated Human HSD17B13 Detection Antibody | 0.12 mL
  • 100x SA-HRP Conjugate | 0.12 mL
  • Assay Buffer | 60 mL
  • Detection Antibody Diluent | 12 mL
  • Wash Buffer Concentrate 20X | 60 mL
  • TMB Substrate | 12 mL
  • Stop Solution | 12 mL
• Background: 17β-Hydroxysteroid dehydrogenase type 13 (HSD17B13) is an enzyme that in humans is encoded by the HSD17B13 gene. HSD17B13 is a 300-amino-acid protein that is localized on lipid droplet. Its expression is highly restricted to the liver, specifically in hepatocytes but not other cell types in the liver. HSD17B13 is involved in various metabolic processes, including steroid hormones, fatty acids, cholesterol, and bile acids. HSD17B13 has been reported to catalyze multiple substrates including steroids, bioactive lipids including leukotriene B4 and 12(R)-hydroxyeicosatetraenoic acid, and retinol. HSD17B13 enzymatic activity attributes to hepatic cell injury, inflammation, and fibrosis, chronically leading to the development of alcoholic liver disease (ALD), nonalcoholic steatohepatitis (NASH), cirrhosis, and even hepatocellular carcinoma (HCC). HSD17B13 expression is significantly upregulated in patients and mice with non-alcoholic fatty liver disease (NAFLD). Hepatic overexpression of HSD17B13 promotes lipid accumulation in the liver. It has potential as a biomarker of advanced liver disease, such as non-alcoholic steatohepatitis (NASH) and liver cancer.
• Gene Symbol: HSD17B13
• Locus ID: 345275
• Shipping: Blue Ice