3.5 hours incubations; 1 hour washing and analyzing samples
Signal
Colorimetric
Curve Range
46.88pg/ml-3000pg/ml
Sample Type
Human serum, plasma and other biological fluids.
Sample Volume
100µl
Specificity
This kit is used for quantitative detection of Human AMH
Sensitivity
12.9pg/ml
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Interference
No significant interference observed with available related molecules.
Components
AMH Antibody Coated 96-well Plate in foil pouch with desiccant | 1 plate
Recombinant Human AMH Standard (150ng/ml) | 0.1 mL
Biotinylated Human AMH Detection Antibody | 0.12 mL
100x Peroxidase Conjugate | 0.12 mL
Assay Buffer | 30 mL
Sample Diluent | 15 mL
Wash Buffer Concentrate 20X | 60 mL
TMB Substrate | 12 mL
Stop Solution | 12 mL
Background
Anti-Müllerian hormone (AMH), also known as Müllerian-inhibiting hormone (MIH) or Müllerian inhibiting substance (MIS). Wild-type human AMH protein is synthesized as a disulfide-linked dimer of two identical 70-kDa polypeptides, which undergoes proteolytic processing to generate a 110-kDa N-terminal dimer and a bioactive 25-kDa TGF-β-like C-terminal dimer. The N-terminal dimer interacts with C-terminal dimer via non-covalent bond to form a bioactive complex that binds to its Type 2 receptor AMHR2. AMH is critical to sex differentiation during fetal development and is a predictor for ovarian response in in vitro fertilization and useful in fertility assessment. In addition, AMH has been shown to be a biomarker for diagnosis as well as monitoring for recurrences of ovarian tumors of granulosa cell origin, and a biomarker of polycystic ovary syndrome and Turner Syndrome.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.