Human IL6 ELISA KIT (1 x 96 wells)

CAT#: EA200005

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Monitor Inflammation with Human IL-6 ELISA Kit. Sensitivity: 1.28 pg/mL. Validated for Cell Culture Supernates, Serum, plasma. High precision and reproducibility.



Special Offer: 30% off this product. Use code "ELISA30".

USD 495.00

In Stock*

Size
    • 1 x 96 wells

Product images

Specifications

Product Data
Format 8x12 divisible strips
Assay Type Sandwich ELISA kit of Quantitative Detection for Human IL-6
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 4.69pg/ml-300pg/ml
Sample Type Human serum, plasma and culture supernates.
Sample Volume 100µl
Specificity This kit is used for quantitative detection of Human IL-6
Sensitivity 1.28pg/ml
Reactivities Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • IL-6 Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate
  • Recombinant Human IL-6 Standard (60ng/ml)|0.1 mL
  • 100x Biotinylated HumanIL-6 Detection Antibody|0.12 mL
  • 100x Peroxidase Conjugate|0.12 mL
  • Assay Buffer|30 mL
  • Sample Diluent|12 mL
  • Wash Buffer Concentrate 20X|60 mL
  • Substrate|12 mL
  • Stop Solution|10 mL
Background Interleukin 6 (IL-6) is a cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, and bone metabolism. The human IL-6 shares 41% aa sequence identity with mouse and rat IL-6. IL-6 is produced by lymphoid and non-lymphoid cells and by normal and transformed cells, including T cells, monocyte/macrophages, fibroblasts, hepatocytes, vascular endothelial cells, cardiac myxomas, bladder cell carcinomas, myelomas, astrogliomas and glioblastomas. The production of IL-6 in these cells is regulated, either positively or negatively, by a variety of signals including mitogens, antigenic stimulation, LPS, IL-1, TNF, PDGF and viruses. IL-6 is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha (IL-6 Rα). The effects of IL-6 on different cells are numerous and varied. The effect on B cells is stimulation of differentiation and antibody secretion. IL-6 affects T cells, acting as a co-stimulant with sub-optimal concentrations of PHA or Con A to stimulate IL-2 production and IL-2 receptor expression. IL-6 is implicated in a wide variety of inflammation-associated disease states, including susceptibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. In addition, it has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. Normal human circulating IL-6 is in the 1 pg/mL range, with elevations in certain cancers, inflammatory diseases and after surgery.
Gene Symbol IL6
Gene ID 3569
Standard Curve
Data image for Human IL-6 ELISA kit.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.