3.5 hours incubations; 1 hour washing and analyzing samples
Signal
Colorimetric
Curve Range
31pg/ml-2000pg/ml
Sample Type
Human serum, plasma and urine.
Sample Volume
100µl
Specificity
This kit is used for quantitative detection of Human NGAL
Sensitivity
11pg/ml
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Interference
No significant interference observed with available related molecules.
Components
NGAL Antibody Coated 96-well Plate in foil pouch with desiccant | 1 plate
Recombinant Human NGAL Standard (100ng/ml) | 0.1 mL
HRP conjugated NGAL Detection Antibody | 0.12 mL
Assay Buffer | 60 mL
Wash Buffer Concentrate 20X | 60 mL
TMB Substrate | 12 mL
Stop Solution | 12 mL
Background
Neutrophil gelatinase-associated lipocalin (NGAL) also called Lipocalin-2 (LCN2), is a protein that in humans is encoded by the LCN2 gene. NGAL belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. NGAL plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. This protein is thought to be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. NGAL can also be used as predictive biomarker for chronic kidney disease, contrast induced nephropathy, and delayed graft function following kidney transplantation.
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