Human Cotinine ELISA kit

CAT#: EA100901

Human Cotinine ELISA kit

Specifications

Product Data

• Format: 8x12 divisible strips
• Assay Type: Solid Phase Competitive ELISA
• Assay Length: 3 hours
• Signal: Colorimetric
• Curve Range: 5-100 ng/ml
• Sample Type: Serum, Urine
• Sample Volume: 10 µl/well
• Specificity: This kit is used for quantitative detection human Cotinine
• Sensitivity: 5 ng/ml
• Reactivities: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Interference: No significant interference observed with available related molecules.
• Components:
  • 1. Microwells coated with polyclonal Ab to Cotinine: 12x8x1
  • 2. Standard Set (ready to use): 0.5 ml
  • 3. Cotinine HRP Enzyme Conjugate (ready to use): 12 ml
  • 4. TMB Substrate (ready to use): 12 ml
  • 5. Stop Solution (ready to use): 12 ml
  • 6. Wash Buffer 20x (1 bottle): 25 ml
• Background: Exposure to tobacco smoke can be detected by measuring nicotine and its metabolites. Nicotine has a short half life and is not used as a marker for tobacco smoke exposure. Cotinine due to its longer half life has been used in research as a reliable marker for smoking status and smoking cessation studies. The Cotinine Direct ELISA Kit is designed for the detection Cotinine in serum and urine. It can also be adapted for other fluids.