Human Cotinine ELISA kit

Snippet: As a biological marker of tobacco exposure, plasma concentrations of cotinine, a metabolite of nicotine combustion, were determined by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (Origene, Cat. EA100901;Rockville, MD, USA)

Snippet: samples were spun (3000 g for 10 min), and then the plasmas were collected and frozen at − 80 °C until analysis. Cotinine levels of the plasmas were measured with an ELISA kit (Cat# EA100901, OriGene, Rockville, MD), following the manufacturer's instructions. Culture of human podocytes Human podocytes were cultured as previously reported (Lan et al. 2016;Wen et al. 2019). Briefly, immort

Snippet: … All participants self-reported a least 6 months of smoking cessation prior to study enrolment, which was continued throughout the study. Serum cotinine was measured at each exposure visit to verify abstinence from smoking, which remained 10ng/mL for all participants (Cotinine ELISA Kit, OriGene Technologies, Inc, catalog# EA100901)

Snippet: ere 5.6% and 7.5%, respectively. Serum levels of cotinine, a metabolite of nicotine and the primary biomarker for the determination of tobacco exposure,22 were evaluated by a commercial immunoassay (Origene, Rockville, MD), and values are expressed as ng/mL;intra‐ and interassay coefficients of variation were;Serum levels of cotinine, a metabolite of nicotine and the primary biomarker for the det...