Rat IL-6 ELISA Kit (96-well)
| Product Data | |
| Species | Rat |
|---|---|
| Format | 96-well strip plate |
| Assay Type | Solid Phase Sandwich ELISA |
| Assay Length | 3 hour |
| Signal | Colorimetric |
| Curve Range | 0.13 - 8.0 ng/mL |
| Sample Type | Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) |
| Sample Volume | 20 uL |
| Specificity | Natural and recombinant Rat IL-6 Ligand |
| Sensitivity | 60pg/mL |
| Reactivity | Rat |
| Interference | No significant interference observed with available related molecules. |
| Components |
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| Background | Interleukin 6 (IL-6) is a multifunctional cytokine that plays important roles in host defense, acute phase reactions, immune responses, nerve cell functions and hematopoiesis . IL-6 is a prototypic member of the IL-6 superfamily of cytokines that share gp130 as a component required for signal transduction . The rat, mouse and human IL-6 cDNAs have been cloned . Rat IL-6 cDNA encodes a 211 amino acid (aa) residue precursor polypeptide with a hydrophobic signal peptide that is cleaved to generate the 187 aa residue mature protein. At the protein sequence level, there is approximately 39% identity between rat and human, and 87% identity between mouse and rat IL-6 . IL-6 is expressed by a variety of normal and transformed lymphoid and non-lymphoid cells. The production of IL-6 is up-regulated by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines and viruses. IL-4, IL-10 and IL-13 inhibit IL-6 expression in monocytes. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections,trauma, autoimmune diseases, inflammations and malignancies . |
| Gene Symbol | Il6 |
| Locus ID | 24498 |
| Standard Curve |
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| Shipping | Blue Ice |
| Datasheet | |
| SDS | |