WIBG (PYM1) Mouse Monoclonal Antibody [Clone ID: UMAB207]

CAT#: UM800099CF

Carrier-free (BSA/glycerol-free) WIBG mouse monoclonal antibody,clone UMAB207

Product Images

Immunohistochemical staining of paraffin-embedded normal/normal adjacent breast with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic and some nuclear in normal/normal adjacent breast tissue.

• 

  Immunohistochemical staining of paraffin-embedded breast carcinoma with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

• 

  Immunohistochemical staining of paraffin-embedded thyroid with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

• 

  Immunohistochemical staining of paraffin-embedded pancreas with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

• 

  Immunohistochemical staining of paraffin-embedded kidney with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

• 

  Immunohistochemical staining of paraffin-embedded ovarian cancer with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

• Immunohistochemical staining of paraffin-embedded colon cancer with mouse anti-WIBG clone UMAB207 1:400 of 1mg/mL using HIER TEE pH9.0 [GBI Labs B21-Tris/EDTA HIER]. Expression of WIBG is cytoplasmic.

• Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

• Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-WIBG monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen). (1:500) Dilution: 1:500

• Immunofluorescent staining of HeLa cells using anti-WIBG mouse monoclonal antibody (UM800099, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-WIBG mouse monoclonal antibody (UM800099). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Show all 11 images

Specifications

Product Data

• Clone Name: UMAB207
• Applications: 10k-ChIP, IF, IHC, WB
• Recommended Dilution: IHC 1:100~500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human WIBG (NP_115721) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.5 kDa
• Gene Name: Homo sapiens PYM homolog 1, exon junction complex associated factor (PYM1), transcript variant 1, mRNA.
• Database Link:
  NP_115721
  Entrez Gene 84305 Human
  UniProt ID Q9BRP8
• Background: WIBG (within bgcn homolog (Drosophila)) is a protein-coding gene. Diseases associated with WIBG include mucoepidermoid carcinoma, and tongue cancer. GO annotations related to this gene include ribosome binding and RNA binding.
• Synonyms: PYM; WIBG