Peripherin (PRPH) Mouse Monoclonal Antibody [Clone ID: UMAB202]

CAT#: UM800094CF

Carrier-free (BSA/glycerol-free) PRPH mouse monoclonal antibody,clone UMAB202

Product Images

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, UM800094) (1:200)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, UM800094) (1:200)

• 

  Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, UM800094) (1:200)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, UM800094) (1:200)

• 

  Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, UM800094) (1:200)

• 

  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, UM800094) (1:200)

• Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PRPH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, UM800094) (1:200)

• Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

• Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

• Confocal immunofluoresce image of primary rat neurons labeled with anti-PRPH mouse monoclonal antibody (UM800094, green, 1:50). Actin filaments were labeled with TRICT-Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Show all 10 images

Specifications

Product Data

• Clone Name: UMAB202
• Applications: IF, IHC, WB
• Recommended Dilution: IHC 1:200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRPH (NP_006253) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: Homo sapiens peripherin (PRPH), mRNA.
• Database Link:
  NP_006253
  Entrez Gene 19132 MouseEntrez Gene 24688 RatEntrez Gene 5630 Human
  UniProt ID P41219
• Background: This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis. [provided by RefSeq, Jul 2008]
• Synonyms: NEF4; PRPH1

Reference Data

• Protein Pathways: Amyotrophic lateral sclerosis (ALS)