MRPS7 Mouse Monoclonal Antibody [Clone ID: UMAB156]

CAT#: UM800053CF

Carrier-free (BSA/glycerol-free) MRPS7 mouse monoclonal antibody,clone UMAB156

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRPS7 mouse monoclonal antibody. (UM800053; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-MRPS7 mouse monoclonal antibody. (UM800053; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRPS7 mouse monoclonal antibody. (UM800053; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MRPS7 mouse monoclonal antibody. (UM800053; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MRPS7 mouse monoclonal antibody. (UM800053; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MRPS7 mouse monoclonal antibody. (UM800053; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MRPS7 mouse monoclonal antibody. (UM800053; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-MRPS7 mouse monoclonal antibody. (UM800053; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MRPS7 mouse monoclonal antibody. (UM800053; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MRPS7 mouse monoclonal antibody. (UM800053; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded human composit of placenta using anti-MRPS7 clone UMAB156 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM800053 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in trophoblast cells.

• Immunohistochemical staining of paraffin-embedded human normal brain using anti-MRPS7 clone UMAB156 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. UM800053 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C.

• Western blot analysis of HCT116 cell lysate (35ug) by using anti-MRPS7 monoclonal antibody.

• Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and MRPS7-Knockout HeLa cells (KO, Cat# LC831777) were separated by SDS-PAGE and immunoblotted with anti-MRPS7 monoclonal antibody UM800053 (1:250). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

• Immunofluorescent staining of MCF-7 cells using anti-MRPS7 mouse monoclonal antibody (UM800053, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

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Specifications

Product Data

• Clone Name: UMAB156
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 38-242 of human MRPS7 (NP_057055) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens mitochondrial ribosomal protein S7 (MRPS7), mRNA; nuclear gene for mitochondrial product.
• Database Link:
  NP_057055
  Entrez Gene 51081 Human
  UniProt ID Q9Y2R9
• Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. In the prokaryotic ribosome, the comparable protein is thought to play an essential role in organizing the 3' domain of the 16 S rRNA in the vicinity of the P- and A-sites. Pseudogenes corresponding to this gene are found on chromosomes 8p and 12p. [provided by RefSeq, Jul
• Synonyms: bMRP27a; MRP-S; MRP-S7; RP-S7; RPMS7; S7mt