DDX56 Mouse Monoclonal Antibody [Clone ID: UMAB153]

CAT#: UM800050CF

Carrier-free (BSA/glycerol-free) DDX56 mouse monoclonal antibody,clone UMAB153

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DDX56mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DDX56 mouse monoclonal antibody. (UM800050; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

• Western blot of mouse tissue lysates (20ug) from Spleen. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).

• Immunofluorescent staining of HeLa cells using anti-DDX56 mouse monoclonal antibody (UM800050, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-DDX56 mouse monoclonal antibody (UM800050). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB153
• Applications: 10k-ChIP, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:100~200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 323-547 of human DDX56 (NP_061955) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: DEAD-box helicase 56
• Database Link:
  NP_061955
  Entrez Gene 54606 Human
  UniProt ID Q9NY93
• Background: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]
• Synonyms: DDX21; DDX26; NOH61