HADHSC (HADH) Mouse Monoclonal Antibody [Clone ID: UMAB151]

CAT#: UM800048CF

Carrier-free (BSA/glycerol-free) HADH mouse monoclonal antibody,clone UMAB151

Product Images

Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human tonsil using anti-HADH mouse monoclonal antibody. (UM800048; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Western blot analysis of HEK293 cell lysate (35ug) by using anti-HADH monoclonal antibody.

• Western blot analysis of Jurkat cell lysate (35ug) by using anti-HADH monoclonal antibody.

• Western blot analysis of LOVO cell lysate (35ug) by using anti-HADH monoclonal antibody.

• Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

• Western blot of mouse tissue lysates (20ug) from 6 different tissues (1: Uterus, 2: Brain, 3: Liver, 4: Ovary, 5: Colon, 6: Spleen ). Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-HADH mouse monoclonal antibody (UM800048). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB151
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:100~200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 57-314 of human HADH (NP_005318) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens hydroxyacyl-CoA dehydrogenase (HADH), transcript variant 2, mRNA; nuclear gene for mitochondrial product.
• Database Link:
  NP_005318
  Entrez Gene 15107 MouseEntrez Gene 113965 RatEntrez Gene 3033 Human
  UniProt ID Q16836
• Background: This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq, May 2010]
• Synonyms: HAD; HADH1; HADHSC; HCDH; HHF4; MSCHAD; SCHAD

Reference Data

• Protein Pathways: Butanoate metabolism, Fatty acid elongation in mitochondria, Fatty acid metabolism, Lysine degradation, Metabolic pathways, Tryptophan metabolism, Valine, leucine and isoleucine degradation