Thymidine Phosphorylase (TYMP) Mouse Monoclonal Antibody [Clone ID: UMAB97]

CAT#: UM800027CF

Carrier-free (BSA/glycerol-free) TYMP mouse monoclonal antibody,clone UMAB97

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TYMP mouse monoclonal antibody. (UM800027; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-TYMP mouse monoclonal antibody. (UM800027; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TYMP mouse monoclonal antibody. (UM800027; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-TYMP mouse monoclonal antibody. (UM800027; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TYMP mouse monoclonal antibody. (UM800027; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human tonsil using anti-TYMPmouse monoclonal antibody. (UM800027; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded human tonsil using anti-TYMP clone UMAB97 mouse monoclonal antibody (UM800027) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pre

• Immunohistochemical staining of paraffin-embedded human breast cancer using anti-TYMP clone UMAB97 mouse monoclonal antibody (UM800027) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer us

• Immunohistochemical staining of paraffin-embedded human gastric cancer using anti-TYMP clone UMAB97 mouse monoclonal antibody (UM800027) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer u

• Immunohistochemical staining of paraffin-embedded human liver cancer using anti-TYMP clone UMAB97 mouse monoclonal antibody (UM800027) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer usi

• Immunohistochemical staining of paraffin-embedded human placenta using anti-TYMP clone UMAB97 mouse monoclonal antibody (UM800027) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using p

• Western Blot analysis of A498, RPMI-8226 and LOX-IMVI cell lysates (35ug) by using anti-TYMP monoclonal antibody (Clone UMAB97)

• Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.

• Western blot of mouse tissue lysates (20ug) from Brain. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).

• Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and TYMP-Knockout HeLa cells (KO, Cat# LC831279) were separated by SDS-PAGE and immunoblotted with anti-TYMP monoclonal antibody UM800027 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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Specifications

Product Data

• Clone Name: UMAB97
• Applications: IHC, WB
• Recommended Dilution: IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 311-482 of human TYMP (NP_001944) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.8 kDa
• Gene Name: Homo sapiens thymidine phosphorylase (TYMP), transcript variant 2, mRNA.
• Database Link:
  NP_001944
  Entrez Gene 1890 Human
  UniProt ID P19971
• Background: This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]
• Synonyms: ECGF; ECGF1; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; TP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Bladder cancer, Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism