CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: UMAB60]

CAT#: UM500051CF

Carrier-free (BSA/glycerol-free) FCGR2A mouse monoclonal antibody, clone UMAB60

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FCGR2A mouse monoclonal antibody. (UM500051; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-FCGR2A mouse monoclonal antibody. (UM500051; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FCGR2A mouse monoclonal antibody. (UM500051; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-FCGR2A mouse monoclonal antibody. (UM500051; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FCGR2Amouse monoclonal antibody. (UM500051; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FCGR2A mouse monoclonal antibody. (UM500051; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FCGR2A mouse monoclonal antibody. (UM500051; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FCGR2A mouse monoclonal antibody. (UM500051; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-FCGR2A mouse monoclonal antibody. (UM500051; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (RC227758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A (1:500).

• Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-FCGR2A mouse monoclonal antibody (UM500051). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Show all 12 images

Specifications

Product Data

• Clone Name: UMAB60
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.7 kDa
• Gene Name: Fc fragment of IgG receptor IIa
• Database Link:
  NP_067674
  Entrez Gene 2212 Human
  UniProt ID P12318
• Background: This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]
• Synonyms: CD32; CD32A; CDw32; FCG2; FcGR; FCGR2; FCGR2A1; IGFR2

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Fc gamma R-mediated phagocytosis, Systemic lupus erythematosus