DEF6 Mouse Monoclonal Antibody [Clone ID: UMAB51]

CAT#: UM500046CF

Carrier-free (BSA/glycerol-free) DEF6 mouse monoclonal antibody,clone UMAB51

Product Images

Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-DEF6 mouse monoclonal antibody. (UM500046; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DEF6 mouse monoclonal antibody. (UM500046; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-DEF6 mouse monoclonal antibody. (UM500046; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DEF6 mouse monoclonal antibody. (UM500046; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DEF6 mouse monoclonal antibody. (UM500046; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human tonsil using anti-DEF6mouse monoclonal antibody. (UM500046; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded mouse small intestine tissue using anti-DEF6 clone UMAB51 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, UM500046 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

• Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-DEF6 clone UMAB51 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, UM500046 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

• Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-DEF6 clone UMAB51 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, UM500046 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

• Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DEF6 monoclonal antibody (Clone UMAB51).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-DEF6 mouse monoclonal antibody (UM500046). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB51
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DEF6(NP_071330) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.7 kDa
• Gene Name: Homo sapiens DEF6 guanine nucleotide exchange factor (DEF6), mRNA.
• Database Link:
  NP_071330
  Entrez Gene 23853 MouseEntrez Gene 309642 RatEntrez Gene 50619 Human
  UniProt ID Q9H4E7
• Background: DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]). [supplied by OMIM, Mar 2008]
• Synonyms: IBP; SLAT; SWAP70L

Reference Data

• Protein Families: Druggable Genome