beta Catenin (CTNNB1) Mouse Monoclonal Antibody [Clone ID: UMAB14]

CAT#: UM500014CF

Carrier-free (BSA/glycerol-free) Beta-Catenin (CTNNB1) mouse monoclonal antibody, clone UMAB14

Product Images

Immunohistochemical staining of paraffin-embedded human normal ovary and Adenocarcinoma of the ovary tissues using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB14, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120

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  Immunohistochemical staining of paraffin-embedded human normal lung and lung Adenocarcinoma tissues using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB14, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3mi

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  Immunohistochemical staining of paraffin-embedded Mouse colon tissue using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB14, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CTNNB1 clone UMAB14 mouse monoclonal antibody. HIER ACCEL buffer (B22C-125) (pH8.7) at 110C for 10 min, UM500014 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

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  Immunohistochemical staining of paraffin-embedded mouse brain tissue using anti-CTNNB1 clone UMAB14 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, UM500014 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

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  Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-CTNNB1 clone UMAB14 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, UM500014 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

• Immunohistochemical staining of paraffin-embedded mouse kidney tissue using anti-CTNNB1 clone UMAB14 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, UM500014 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

• Immunohistochemical staining of paraffin-embedded mouse small intestine tissue using anti-CTNNB1 clone UMAB14 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, UM500014 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-beta-catenin mouse monoclonal antibody (Clone UMAB14). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-beta-catenin (Clone UMAB14) very specifically recognizes beta-catenin antigen on OriGene protein microarray chip.

• Flow cytometric Analysis of Hela cells, using anti-CTNNB1 antibody (UM500014), (Red), compared to a nonspecific negative control antibody, (Blue).

• Western analysis of HEK 293T cells without treatment or treated with 30mM LiCl for 16 hours, using anti-beta-catenin antibody (clone UMAB14). Anti-HSP90 (TA500494) was used as internal loading control.

• Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody (Clone UMAB14).

• MDCK cells were treated either with 30mM NaCl as negative control (left) or with GSK-3beta kinase inhibor 30mM LiCl (right) for 48 hrs. Anti-beta-catenin monoclonal antibody (clone UMAB14) was used for immunofluorescent staining.

• HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (RC208947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1 (1:500).

• Western Blot analysis of 10 different human tissue lysates (10ug) by using Anti-β-Catenin monoclonal antibody (Clone UMAB14, 1:500)

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Specifications

Product Data

• Clone Name: UMAB14
• Applications: 10k-ChIP, FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CTNNB1 (NP_001895) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85.3 kDa
• Gene Name: catenin beta 1
• Database Link:
  NP_001895
  Entrez Gene 12387 MouseEntrez Gene 84353 RatEntrez Gene 477032 DogEntrez Gene 574265 MonkeyEntrez Gene 1499 Human
  UniProt ID P35222
• Background: The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.
• Synonyms: armadillo; CTNNB; MRD19

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Focal adhesion, Leukocyte transendothelial migration, Melanogenesis, Pathogenic Escherichia coli infection, Pathways in cancer, Prostate cancer, Thyroid cancer, Tight junction, Wnt signaling pathway