GFAP Mouse Monoclonal Antibody [Clone ID: UMAB5]

CAT#: UM500005CF

Carrier-free (BSA/glycerol-free) GFAP mouse monoclonal antibody, clone UMAB5

Product Images

Immunohistochemical staining of paraffin-embedded Human brain tissue using anti-GFAP mouse monoclonal antibody (Clone UMAB5; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

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  Immunohistochemical staining of paraffin-embedded Human embryonic brain tissue using anti-GFAP mouse monoclonal antibody (Clone UMAB5; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-GFAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM500005) (1:500)

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  Immunohistochemical staining of paraffin-embedded mouse brain tissue using anti-GFAP clone UMAB5 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, UM500005 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (RC204548, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP (1:500).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GFAP monoclonal antibody (Clone UMAB5).

• Western blot analysis of extracts (35ug) from 1 cell line lysate and 1 tissue lysate by using anti-GFAP monoclonal antibody (1:500).

• Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-GFAP monoclonal antibody (clone UMAB5, 1:500)

• Immunofluorescent staining of PC-12 cells using GFAP mouse monoclonal antibody (UM500005, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

• Confocal immunofluoresce image of primary rat neurons labeled with anti-GFAP mouse monoclonal antibody (UM500005, green, 1:100) and with DAPI (blue) for nuclear. Scale bar, 10um.

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Specifications

Product Data

• Clone Name: UMAB5
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:50, IF 1:100
• Reactivities: Human, Rat, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GFAP (NP_002046) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.9 kDa
• Gene Name: glial fibrillary acidic protein
• Database Link:
  NP_002046
  Entrez Gene 14580 MouseEntrez Gene 24387 RatEntrez Gene 2670 Human
  UniProt ID P14136
• Background: This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Synonyms: ALXDRD

Reference Data

• Protein Families: ES Cell Differentiation/IPS