UAP56 (DDX39B) Mouse Monoclonal Antibody [Clone ID: OTI2F11]

SKU TA812665

DDX39B mouse monoclonal antibody,clone OTI2F11

Product Data

• Clone Name: OTI2F11
• Application: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivity: Dog, Human, Monkey, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 319-428 of human DDX39B (NP_004631) produced in E.coli.
• Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 48.8 kDa
• Gene Name: DEAD-box helicase 39B
• Database Link:
  NP_004631
  Q13838
• Background: Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2; the effect of ALYREF/THOC4 is reported conflictingly with [PubMed:23299939] reporting a stimulatory effect. [UniProtKB/Swiss-Prot Function]
• Synonyms: BAT1; D6S81E; UAP56

Reference Data

• Protein Pathways: Spliceosome