CCDC22 Mouse Monoclonal Antibody [Clone ID: OTI2E4]

CAT#: TA811111

CCDC22 mouse monoclonal antibody,clone OTI2E4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CCDC22 (Cat# RC201585, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCDC22 (Cat# TA811111)(1:2000). Positive lysates LY415538 (100ug) and LC415538 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 293T cell line by using anti-CCDC22 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CCDC22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811111) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CCDC22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811111) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CCDC22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811111) (1:500)

Show all 5 images

Specifications

Product Data

• Clone Name: OTI2E4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human CCDC22 (NP_054727) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.6 kDa
• Gene Name: coiled-coil domain containing 22
• Database Link:
  NP_054727
  Entrez Gene 54638 MouseEntrez Gene 317381 RatEntrez Gene 28952 Human
  UniProt ID O60826
• Synonyms: CXorf37; JM1; RTSC2