MED19 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2D1]

CAT#: TA810428BM

MED19 mouse monoclonal antibody,clone OTI2D1, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MED19 (Cat# RC206806, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED19 (Cat# TA810428)(1:2000). Positive lysates LY407036 (100ug) and LC407036 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 4 different cell lines and rat liver tissue lysate by using anti-MED19 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MED19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810428) (1:500)

Show all 18 images

Specifications

Product Data

• Clone Name: OTI2D1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human MED19 (NP_703151) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.3 kDa
• Gene Name: mediator complex subunit 19
• Database Link:
  NP_703151
  Entrez Gene 311165 RatEntrez Gene 381379 MouseEntrez Gene 219541 Human
  UniProt ID A0JLT2
• Synonyms: DT2P1G7; LCMR1; MED19AS

Reference Data

• Protein Families: Druggable Genome