ERLIN1 Mouse Monoclonal Antibody [Clone ID: OTI4B1]

CAT#: TA809148

ERLIN1 mouse monoclonal antibody,clone OTI4B1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ERLIN1 (Cat# RC205137, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERLIN1 (Cat# TA809148)(1:2000). Positive lysates LY416643 (100ug) and LC416643 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-ERLIN1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ERLIN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809148) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ERLIN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809148) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ERLIN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809148) (1:2000)

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Specifications

Product Data

• Clone Name: OTI4B1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 107-346 of human ERLIN1(NP_006450) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39 kDa
• Gene Name: ER lipid raft associated 1
• Database Link:
  NP_006450
  Entrez Gene 226144 MouseEntrez Gene 293939 RatEntrez Gene 10613 Human
  UniProt ID O75477
• Synonyms: C10orf69; Erlin-1; KE04; KEO4; SPFH1; SPG62

Reference Data

• Protein Families: Transmembrane