ROS1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1A1]

CAT#: TA805804BM

ROS1 mouse monoclonal antibody, clone OTI1A1 (formerly 1A1), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ROS1 (Cat# RC220652, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROS1(Cat# TA805804).

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ROS1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded HCC78 (left) and HeLa (right) xenograft using TA805804 (0.2ug/ml). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min).

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  Immunohistochemical staining of paraffin-embedded non-small cell lung cancer tissues (case1 was ROS1-positive, Case2 was ROS1-negative) using TA805804 (0.2ug/ml). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min)

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  Immunocytochemistry staining of HCC78 cells using anti-ROS1 mouse monoclonal antibody (TA805804) (Left). The right is HELA cells as negative control.

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Specifications

Product Data

• Clone Name: OTI1A1
• Applications: IF, IHC, WB
• Recommended Dilution: WB(overexpression) 1:1000, WB(endogenous) 1:200, IF/ICC 1:5000-10000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1882-2125 of human ROS1(NP_002935) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ROS proto-oncogene 1, receptor tyrosine kinase
• Database Link:
  NP_002935
  Entrez Gene 6098 Human
  UniProt ID P08922
• Synonyms: c-ros-1; MCF3; ROS

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane