HDAC9 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI7G2]

CAT#: TA805328AM

HDAC9 mouse monoclonal antibody, clone OTI7G2 (formerly 7G2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HDAC9 (Cat# RC215267, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC9(Cat# TA805328). Positive lysates LY415085 (100ug) and LC415085 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-HDAC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805328)

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Specifications

Product Data

• Clone Name: OTI7G2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 181-460 of human HDAC9 (NP_055522) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 65.7 kDa
• Gene Name: histone deacetylase 9
• Database Link:
  NP_055522
  Entrez Gene 79221 MouseEntrez Gene 687001 RatEntrez Gene 9734 Human
  UniProt ID Q9UKV0
• Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined. [provided by RefSeq, Jul 2008]
• Synonyms: HD7; HD7b; HD9; HDAC; HDAC7; HDAC7B; HDAC9B; HDAC9FL; HDRP; MITR

Reference Data

• Protein Families: Druggable Genome, Transcription Factors