PDE1A Mouse Monoclonal Antibody [Clone ID: OTI7D5]

CAT#: TA803629

PDE1A mouse monoclonal antibody, clone OTI7D5 (formerly 7D5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PDE1A (Cat# RC205292, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1A(Cat# TA803629). Positive lysates LY401556 (100ug) and LC401556 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PDE1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803629)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PDE1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803629)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803629)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDE1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803629)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDE1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803629)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PDE1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803629)

Show all 7 images

Specifications

Product Data

• Clone Name: OTI7D5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 259-545 of human PDE1A (NP_005010) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: phosphodiesterase 1A
• Database Link:
  NP_005010
  Entrez Gene 18573 MouseEntrez Gene 81529 RatEntrez Gene 5136 Human
  UniProt ID P54750
• Background: Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]). [supplied by OMIM, Oct 2009]
• Synonyms: CAM-PDE-1A; HCAM-1; HCAM1; HSPDE1A

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism, Taste transduction