Hsp60 (HSPD1) Mouse Monoclonal Antibody [Clone ID: OTI4E4]

CAT#: TA800756

HSPD1 mouse monoclonal antibody,clone OTI4E4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPD1 (RC201281, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPD1. Positive lysates LY404564 (100ug) and LC404564 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 9 different cell lines by using anti-HSPD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:2000).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800756)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800756)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800756)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800756)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800756)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800756)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800756)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800756)

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Specifications

Product Data

• Clone Name: OTI4E4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Monkey, Dog, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HSPD1 (NP_955472) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: heat shock protein family D (Hsp60) member 1
• Database Link:
  NP_955472
  Entrez Gene 15510 MouseEntrez Gene 63868 RatEntrez Gene 478854 DogEntrez Gene 698024 MonkeyEntrez Gene 3329 Human
  UniProt ID P10809
• Background: This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun
• Synonyms: CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: RNA degradation, Type I diabetes mellitus