PPT1 Mouse Monoclonal Antibody [Clone ID: OTI1F10]

CAT#: TA800618

PPT1 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY human PPT1 (RC203278, Middle lane) cDNA or pCMV6-ENTRY mouse PPT1 (MR218476, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPT1. (TA800618, 1:500). The WB shows that the monoclonal antibody TA800618 reacts only with human PPT1 and does not cross react with mouse or rat PPT1

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PPT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PPT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800618)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PPT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800618)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PPT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800618)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PPT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800618)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PPT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA800618)

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Specifications

Product Data

• Clone Name: OTI1F10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 100-306 of human PPT1 (NP_000301) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.2 kDa
• Gene Name: palmitoyl-protein thioesterase 1
• Database Link:
  NP_000301
  Entrez Gene 5538 Human
  UniProt ID P50897
• Background: The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
• Synonyms: CLN1; INCL; PPT

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fatty acid elongation in mitochondria, Lysosome, Metabolic pathways