SLUG (SNAI2) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1G7]

CAT#: TA800196BM

SNAI2 mouse monoclonal antibody,clone 1G7, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAI2 (RC202365, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAI2. Positive lysates LY401070 (100ug) and LC401070 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SNAI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800196)

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  Anti-SNAI2 mouse monoclonal antibody (TA800196) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAI2 (RC202365).

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  HEK293T cells transfected with either RC202365 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAI2 antibody (TA800196), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1G7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SNAI2 (NP_003059) produced in E.coli.
• Formulation: PBS (pH 7.4) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.8 kDa
• Gene Name: snail family transcriptional repressor 2
• Database Link:
  NP_003059
  Entrez Gene 20583 MouseEntrez Gene 25554 RatEntrez Gene 6591 Human
  UniProt ID O43623
• Background: This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq]
• Synonyms: SLUG; SLUGH1; SNAIL2; WS2D

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adherens junction