WIPF1 Rabbit Polyclonal Antibody

CAT#: TA590158

Rabbit Polyclonal WIPF1/WIP Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY WIPF1 (Cat# RC217601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WIPF1(Cat# TA590158). Positive lysates LY401163 (100ug) and LC401163 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-WIPF1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-WIPF1 rabbit polyclonal antibody. (TA590158)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DNA immunization. This antibody is specific for the Middle Region of the target protein.
• Formulation: 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
• Concentration: 1.2mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: WAS/WASL interacting protein family member 1
• Database Link:
  NP_003378
  Entrez Gene 215280 MouseEntrez Gene 7456 Human
  UniProt ID O43516
• Background: WIPF1 is involved in the organization of the actin cytoskeleton. The protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these WIPF1 and Wiskott-Aldrich syndrome protein may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene. WIPF1 binds to Wiskott-Aldrich syndrome protein, profilin and actin. It is involved with NCK1 and GRB2 in the recruitment and activation of N-WASP and may be involved in regulating the subcellular localization of N-WASP, resulting in the disassembly of stress fibers in filopodia formation.
• Synonyms: PRPL-2; WAS2; WASPIP; WIP
• Note: This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT