IL1 alpha (IL1A) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2F8]

CAT#: TA506849AM

IL1A mouse monoclonal antibody,clone 2F8, Biotinylated

Product Images

Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody (1:200).

• 

  HEK293T cells lysate (5 ug, left lane) and full length human recombinant protein of human IL1A (NP_000566) produced in HEK293T cell (5 ug, right lane)were separated by SDS-PAGE and immunoblotted with anti-IL1A.

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506849)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506849)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506849)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506849)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-IL1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506849)

• Anti-IL1A mouse monoclonal antibody (TA506849) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1A (RC202084).

Show all 8 images

Specifications

Product Data

• Clone Name: OTI2F8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IL1A(NP_000566) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.4 kDa
• Gene Name: interleukin 1 alpha
• Database Link:
  NP_000566
  Entrez Gene 3552 Human
  UniProt ID P01583
• Synonyms: IL-1A; IL1; IL1-ALPHA; IL1F1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, Prion diseases, Type I diabetes mellitus