DRP1 (DNM1L) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3F4]

CAT#: TA506112AM

DNM1L mouse monoclonal antibody,clone 3F4, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNM1L (RC221708, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNM1L. Positive lysates LY402138 (100ug) and LC402138 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNM1L monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506112)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506112)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506112)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-DNM1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506112)

• Anti-DNM1L mouse monoclonal antibody (TA506112) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNM1L (RC221708).

• Immunofluorescent staining of HeLa cells using anti-DNM1L mouse monoclonal antibody (TA506112).

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Specifications

Product Data

• Clone Name: OTI3F4
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DNM1L(NP_036192) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81.7 kDa
• Gene Name: dynamin 1 like
• Database Link:
  NP_036192
  Entrez Gene 74006 MouseEntrez Gene 114114 RatEntrez Gene 10059 Human
  UniProt ID O00429
• Background: The protein encoded by this gene is a member of the dynamin superfamily of GTPases. Members of the dynamin-related subfamily, including the S. cerevisiae proteins Dnm1 and Vps1, contain the N-terminal tripartite GTPase domain but do not have the pleckstrin homology or proline-rich domains. This protein establishes mitochondrial morphology through a role in distributing mitochondrial tubules throughout the cytoplasm. The gene has 3 alternatively spliced transcripts encoding different isoforms. These transcripts are alternatively polyadenylated. [provided by RefSeq, Jul 2008]
• Synonyms: DLP1; DRP1; DVLP; DYMPLE; EMPF; EMPF1; HDYNIV

Reference Data

• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis