RNF39 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5E10]

CAT#: TA505912AM

RNF39 mouse monoclonal antibody,clone 5E10, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RNF39 (Cat# RC219332, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF39(Cat# TA505912). Positive lysates LY410747 (100ug) and LC410747 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RNF39 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RNF39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505912)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RNF39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505912)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RNF39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505912)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RNF39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505912)

• Anti-RNF39 mouse monoclonal antibody (TA505912) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF39 (RC219332).

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Specifications

Product Data

• Clone Name: OTI5E10
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200, IHC 1:150, IF 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RNF39(NP_079512) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.3 kDa
• Gene Name: ring finger protein 39
• Database Link:
  NP_079512
  Entrez Gene 171387 RatEntrez Gene 386454 MouseEntrez Gene 488344 DogEntrez Gene 712215 MonkeyEntrez Gene 80352 Human
  UniProt ID Q9H2S5
• Synonyms: HZF; HZFW; LIRF

Reference Data

• Protein Families: Druggable Genome