PFDN3 (VBP1) Mouse Monoclonal Antibody [Clone ID: OTI2C2]

CAT#: TA504903

VBP1 mouse monoclonal antibody, clone OTI2C2 (formerly 2C2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VBP1 (RC208482, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VBP1. Positive lysates LY418727 (100ug) and LC418727 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-VBP1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504903)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504903)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504903)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-VBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504903)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504903)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-VBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504903)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504903)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-VBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504903)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-VBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504903)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-VBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504903)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504903)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-VBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504903)

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Specifications

Product Data

• Clone Name: OTI2C2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VBP1(NP_003363) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.4 kDa
• Gene Name: VHL binding protein 1
• Database Link:
  NP_003363
  Entrez Gene 22327 MouseEntrez Gene 7411 Human
  UniProt ID P61758
• Background: The protein encoded by this gene interacts with the Von Hippel-Lindau protein to form an intracellular complex. Because it functions as a chaperone protein, it is suspected that it may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm. [provided by RefSeq]
• Synonyms: HIBBJ46; PFD3; PFDN3; VBP-1

Reference Data

• Protein Families: Druggable Genome