ATRIP Mouse Monoclonal Antibody [Clone ID: OTI5E7]

CAT#: TA504641

ATRIP mouse monoclonal antibody, clone OTI5E7 (formerly 5E7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ATRIP (Cat# RC223562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATRIP(Cat# TA504641). Positive lysates LY408977 (100ug) and LC408977 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-ATRIP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ATRIP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504641)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ATRIP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504641)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ATRIP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504641)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATRIP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504641)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ATRIP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504641)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATRIP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504641)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ATRIP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504641)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ATRIP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504641)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ATRIP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504641)

• Anti-ATRIP mouse monoclonal antibody (TA504641) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATRIP (RC223562).

• HEK293T cells transfected with either RC223562 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATRIP antibody (TA504641), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-ATRIP antibody (TA504641), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-ATRIP antibody (TA504641), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI5E7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 42-309 of human ATRIP(NP_569055) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1.13 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85.7 kDa
• Gene Name: ATR interacting protein
• Database Link:
  NP_569055
  Entrez Gene 608504 DogEntrez Gene 84126 Human
  UniProt ID Q8WXE1
• Background: The product of this gene is an essential component of the DNA damage checkpoint, and binds to single-stranded DNA coated with replication protein A that accumulates at sites of DNA damage. The encoded protein interacts with the ataxia telangiectasia and Rad3 related protein, a checkpoint kinase, resulting in accumulation of the kinase at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: DKFZp762J2115; FLJ12343; MGC20625; MGC21482; MGC26740