ALOX15 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3G8]

CAT#: TA504250BM

ALOX15 mouse monoclonal antibody, clone OTI3G8 (formerly 3G8), HRP conjugated

Product Images

Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human ALOX15 plasmid (RC206621, lane 2), mouse ALOX15 plasmid (MR226258, lane 3) using anti-ALOX15 antibody TA504250 (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

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  Immunohistochemical staining of paraffin-embedded human lung tissue within the normal limits using anti-ALOX15 mouse monoclonal antibody (TA504250). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 minutes (1:150).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

• Anti-ALOX15 mouse monoclonal antibody (TA504250) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALOX15 (RC206621).

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Specifications

Product Data

• Clone Name: OTI3G8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Rat, Monkey
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.6 kDa
• Gene Name: arachidonate 15-lipoxygenase
• Database Link:
  NP_001131
  Entrez Gene 81639 RatEntrez Gene 709212 MonkeyEntrez Gene 246 Human
  UniProt ID P16050
• Synonyms: 12-LOX; 15-LOX-1; 15LOX-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Linoleic acid metabolism, Metabolic pathways