GIRK1 (KCNJ3) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1G3]

CAT#: TA504197AM

KCNJ3 (GIRK1 ) mouse monoclonal antibody, clone OTI1G3 (formerly 1G3), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KCNJ3 (RC205322, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KCNJ3. Positive lysates LY400811 (100ug) and LC400811 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KCNJ3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504197)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-KCNJ3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504197)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-KCNJ3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504197)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KCNJ3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504197)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KCNJ3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504197)

• Anti-KCNJ3 mouse monoclonal antibody (TA504197) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KCNJ3 (RC205322).

• HEK293T cells transfected with either RC205322 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KCNJ3 antibody (TA504197), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-KCNJ3 antibody (TA504197), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1G3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 279-501 of human KCNJ3(NP_002230) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.4 kDa
• Gene Name: potassium inwardly rectifying channel subfamily J member 3
• Database Link:
  NP_002230
  Entrez Gene 16519 MouseEntrez Gene 50599 RatEntrez Gene 3760 Human
  UniProt ID P48549
• Background: Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex. [provided by RefSeq]
• Synonyms: GIRK1; KGA; KIR3.1

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane