Nucleobindin 1 (NUCB1) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1A5]

CAT#: TA503868BM

NUCB1 (Nucleobindin 1) mouse monoclonal antibody, clone OTI1A5 (formerly 1A5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NUCB1 (Cat# RC201786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUCB1 (Cat# TA503868). Positive lysates LY416822 (100ug) and LC416822 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-NUCB1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NUCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503868)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NUCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503868)

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  Anti-NUCB1 mouse monoclonal antibody (TA503868) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUCB1 (RC201786).

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  HEK293T cells transfected with either RC201786 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NUCB1 antibody (TA503868), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1A5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NUCB1(NP_006175) produced in HEk293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.7 kDa
• Gene Name: nucleobindin 1
• Database Link:
  NP_006175
  Entrez Gene 84595 RatEntrez Gene 4924 Human
  UniProt ID Q02818
• Background: This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq, Jun 2010]
• Synonyms: CALNUC; NUC