PSMD2 Mouse Monoclonal Antibody [Clone ID: OTI1G6]

CAT#: TA503217

PSMD2 (Proteasome 26S S2) mouse monoclonal antibody, clone OTI1G6 (formerly 1G6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PSMD2 (Cat# RC203204, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD2(Cat# TA503217). Positive lysates LY419097 (100ug) and LC419097 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD2 monoclonal antibody.

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503217)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503217)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503217)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503217)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503217)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503217)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503217)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PSMD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503217)

• Anti-PSMD2 mouse monoclonal antibody (TA503217) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMD2 (RC203204).

Show all 11 images

Specifications

Product Data

• Clone Name: OTI1G6
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PSMD2(NP_002799) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.7 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 100 kDa
• Gene Name: proteasome 26S subunit ubiquitin receptor, non-ATPase 2
• Database Link:
  NP_002799
  Entrez Gene 21762 MouseEntrez Gene 287984 RatEntrez Gene 478654 DogEntrez Gene 705035 MonkeyEntrez Gene 5708 Human
  UniProt ID Q13200
• Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. [provided by RefSeq]
• Synonyms: P97; RPN1; S2; TRAP2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Proteasome