ILVBL Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1A12]

CAT#: TA503080BM

ILVBL mouse monoclonal antibody, clone OTI1A12 (formerly 1A12), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ILVBL (RC203987, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ILVBL. Positive lysates LY416389 (100ug) and LC416389 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503080)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503080)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503080)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503080)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503080)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503080)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503080)

• HEK293T cells transfected with either RC203987 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ILVBL antibody (TA503080), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-ILVBL antibody (TA503080), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-ILVBL antibody (TA503080), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1A12
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ILVBL (NP_006835) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67.7 kDa
• Gene Name: ilvB acetolactate synthase like
• Database Link:
  NP_006835
  Entrez Gene 362843 RatEntrez Gene 10994 Human
  UniProt ID A1L0T0
• Background: The protein encoded by this gene shares similarity with several thiamine pyrophosphate-binding proteins identified in bacteria, yeast, and plants. The highest degree of similarity is found with bacterial acetolactate synthases (AHAS), which are enzymes that catalyze the first step in branched-chain amino acid biosynthesis. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: 209L8; AHAS; ILV2H

Reference Data

• Protein Families: Transmembrane