OSGEP Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI9E3]

CAT#: TA503002BM

OSGEP mouse monoclonal antibody, clone OTI9E3 (formerly 9E3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY OSGEP (Cat# RC207089, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSGEP(Cat# TA503002). Positive lysates LY402616 (100ug) and LC402616 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OSGEP monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-OSGEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503002)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-OSGEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503002)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-OSGEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503002)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-OSGEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503002)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-OSGEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503002)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-OSGEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503002)

• Flow cytometric Analysis of Hela cells, using anti-OSGEP antibody (TA503002), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-OSGEP antibody (TA503002), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI9E3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Dog, Rat, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-335 of human OSGEP(NP_060277) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.2 kDa
• Gene Name: O-sialoglycoprotein endopeptidase
• Database Link:
  NP_060277
  Entrez Gene 66246 MouseEntrez Gene 290028 RatEntrez Gene 100687555 DogEntrez Gene 55644 Human
  UniProt ID Q9NPF4
• Synonyms: GCPL1; KAE1; OSGEP1; PRSMG1

Reference Data

• Protein Families: Druggable Genome, Protease