PNMT Mouse Monoclonal Antibody [Clone ID: OTI1D2]

CAT#: TA502822S

PNMT mouse monoclonal antibody, clone OTI1D2 (formerly 1D2)

Product images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PNMT (Cat# RC206586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNMT (Cat# TA502822). Positive lysates LY400946 (100ug) and LC400946 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PNMT monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-PNMT monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502822)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502822)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502822)

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  Anti-PNMT mouse monoclonal antibody (TA502822) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PNMT (RC206586).

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Specifications

Product Data

• Clone Name: OTI1D2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivity: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PNMT(NP_002677) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.7 kDa
• Gene Name: phenylethanolamine N-methyltransferase
• Database Link:
  NP_002677
  Entrez Gene 18948 MouseEntrez Gene 24661 RatEntrez Gene 491023 DogEntrez Gene 697444 MonkeyEntrez Gene 5409 Human
  UniProt ID P11086
• Background: The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq]
• Synonyms: PENT; PNMTase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tyrosine metabolism